Thermo Scientific™ EZ-Link™ Amine-PEG11-Biotin

Description

• Thermo Scientific™ EZ-Link Amine-PEG11-Biotin is a biotinylation reagent containing a long 11-unit polyethylene glycol (PEG) spacer arm and a terminal primary amine group for conjugation and protein labeling with EDC or other crosslinkers
• EZ-Link Amine-PEGn-Biotin reagents are water-soluble, polyethylene glycol (PEG)-containing reagents with terminal primary amines (NH 2 )
• The PEG spacer arm is hydrophilic and confers greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers
• The amine group of these reagents can be reacted with carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No
• 22980), a water-soluble carbodiimide crosslinker
• EDC activates carboxyl groups to bind to the NH 2 group of the amine-biotin, forming an amide bond
• Highlights: Biotin – label macromolecules for detection or affinity purification with avidin and streptavidin Amine-activated – primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and improve the solubility of labeled antibodies Spacer arm – the reach between the point of attachment and the biotin affinity tag is more than 50 angstroms, minimizing steric hindrance for the biotin binding-function Specifications: Chemical name (simplified): biotinyl-undecaoxapentatriacontanediamine Chemical formula: C 34 H 66 N 4 O 13 S Molecular weight: 770.97 Spacer arm: 53.2 angstroms Form: white to off-white solid Solubility: at least 25mg/mL in water or buffer