Thermo Scientific™ Immobilized Ficin (Agarose Resin)

Description

• Thermo Scientific™ Pierce Immobilized Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilized onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1
• Ficin, which is similar to papain, is a nonspecific, sulfhydryl protease isolated from fig latex
• The molecular weight of Ficin is approximately 25,000 and it is effective at pH 4-9.5 with an optimum pH of 6.5
• When IgG molecules are incubated with ficin in the presence of cysteine, one or more peptide bonds in the hinge region are split, producing either Fab fragments or F(ab')2 fragments
• Mouse IgG1 is notably difficult to digest into functional Fab or F(ab')2 fragments using papain or pepsin, however, yields and immunoreactivity are better when digesting mouse IgG1 with ficin
• Immobilized Ficin was specifically designed for cleavage of mouse IgG1 into or Fab or F(ab')2 fragments, and is advantageous because it virtually eliminates autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the ficin or controlling the flow of sample over an immobilized ficin column
• Highlights: Can generate both Fab and F(ab')2 fragments from mouse IgG1 Reaction can be easily controlled Antibody fragments are free of autodigestion products Ficin contamination into antibody fragments is eliminated