MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction NMR Medium-15N

Description

• Autoinduction method is based on the differential metabolism of carbon sources to promote growth to high cell density Automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG) Includes four concentrated sterile solutions: OnEx NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis OnEx Solution 3 provides magnesium for maximal cell density OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels