MilliporeSigma™ Novagen™ Overnight Express™ Autoinduction NMR Medium -15N13C

Description

• Automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG) Includes four concentrated sterile solutions: OnEx Dual-NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by autoinduction OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis OnEx Solution 3 provides magnesium for maximal cell density OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels