7076144
Invitrogen™ Phosphate Sensor
Phosphate Sensor
Manufacturer: Fischer Scientific
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For Use With (Equipment)
Microplate Reader
For Use With (Application)
Fluorescence Intensity
Content And Storage
Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.
Label or Dye
MDCC
Format
384-well plate
Readout
Kinetic
Target
GTPases, ATPases, Phosphatases, Phosphodiesterases
Assay
Phosphatase Assay
Detection Method
Fluorescence
Product Type
Phosphate Sensor
Excitation/Emission
430⁄450
Shipping Condition
Dry Ice
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Description
- The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode
- This product is a purified form of recombinant E
- coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment
- Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence
- The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green
- This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.