PV4406

Invitrogen™ Phosphate Sensor

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 PV4406-Each-of-1 In Stock ₹ 41,239.92

PV4406 - Each of 1

₹ 41,239.92

In Stock

Quantity

1

Base Price: ₹ 41,239.92

GST (18%): ₹ 7,423.186

Total Price: ₹ 48,663.106

For Use With (Equipment)

Microplate Reader

For Use With (Application)

Fluorescence Intensity

Content And Storage

Phosphate Sensor is supplied as a 500 μM solution in 10 mM Tris (pH 7.6) and 50 mM NaCl. Store product at -80°C.

Label or Dye

MDCC

Quantity

10 nmol

Excitation/Emission

430⁄450

Shipping Condition

Dry Ice

Target

GTPases, ATPases, Phosphatases, Phosphodiesterases

Assay

Phosphatase Assay

Detection Method

Fluorescence

Product Type

Phosphate Sensor

Format

384-well plate

Readout

Kinetic

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Description

  • The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode
  • This product is a purified form of recombinant E
  • coli phosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment
  • Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.1μM), resulting in a large increase in fluorescence
  • The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green
  • This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.