7111287

Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit

Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit is two-component, high-precision, detergent-compatible assay optimized to measure (at 480 nm) total protein concentration compared to protein standard curve of known concentrations.

Manufacturer: Fischer Scientific

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Assay Range

20 to 2000 μg/mL

Assay

Rapid Gold BCA Assay

Product Line

Pierce™

Specificity

Not Target-Specific

For Use With (Equipment)

Spectrophotometer, Microplate Reader

Content And Storage

Store in refrigerator (2–8°C).

Detection Method

Colorimetric

Product Type

Protein Quantitation Assay

For Use With (Application)

Solution-based Detection, Absorbance

Shipping Condition

Wet Ice

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Description

  • A fast, two-component, high-precision, detergent-compatible assay optimized to determine total protein concentration Can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications Assay maintains the key characteristics of the traditional BCA assay but allows a fast time and room temperature incubation equal to dye-binding methods Provides comparable accuracy but with a 5 min., room temperature incubation and measured at 480 nm This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results Exhibits similar protein-to-protein variation as traditional BCA Protein Assay and less protein-to-protein variation than dye-binding protein assay methods (Bradford) Unaffected by typical concentrations of most ionic and non-ionic detergents Linear working range for BSA of 20 to 2000 μg/mL Store in refrigerator at 2°C to 8°C