Thermo Scientific™ Pierce™ BCA Protein Assay Reagent A

Description

• Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide
• Additional Information and Online Ordering A component of the Pierce BCA Protein Assay Kit Kit is a two-component, high-precision, detergent-compatible assay that is used for total protein concentration determination compared to a protein standard Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy Estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm Exhibits less protein-to-protein variation than dye-binding methods (Bradford) Unaffected by typical concentrations of most ionic and non-ionic detergents Assay has a 30 min
• incubation; much easier and four times faster than classical Lowry methods Linear working range for BSA of 20 to 2000 μg/mL Detect down to 5 μg/mL with the enhanced protocol Store at room temperature