Thermo Scientific™ Pierce™ Quantitative Peroxide Assay Kit (Lipid)

Description

• Detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange reagent with Thermo Scientific™ Pierce Quantitative Peroxide Assay Kits
• The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (lipid-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent
• This lipid-compatible peroxide assay kit detects peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange
• The kit's formulation may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive
• Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects
• Features of the Quantitative Peroxide Assay Kit: • Peroxides —detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples • Convenient —no proprietary reagents involved but the ready-to-use kit removes the variability, difficulty and added expense of formulating the reagents yourself Applications: • General hydrogen peroxide (H 2 O 2 ) measurement in protein samples • Quantitation of peroxides in detergents • Assessment of the effects of peroxides on cellular activity • Determination of protein glycation; Measurement of peroxide accumulation in lipids In this assay, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH
• With this lipid-compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly
• In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560 nm
• Peroxide levels in test samples can be determined by calculation from the known extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution
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