Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase

Description

• Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit contains horseradish peroxidase enzyme that has been aldehyde-activated with periodate to enable high-efficiency labeling and preparation of protein or antibody conjugates
• Other amine-reactive chemistries, like glutaraldehyde, have a tendency to polymerize and produce lower amounts of viable conjugate
• Sugar residues present on HRP have been oxidized with periodate to produce aldehydes that react with primary amines
• Plus Activated Peroxidase is mixed with the protein to be coupled at a pH compatible with your protein or antibody
• After incubating the reaction for one to two hours, the conjugate linkage is reduced and the activated HRP quenched with ethanolamine
• The conjugate is purified by desalting or by dialysis
• If you are preparing an antibody-HRP conjugate, use the Thermo Scientific Pierce Conjugate Purification Kit (Part No
• 44920) to separate unreacted enzyme from the conjugate
• Highlights: Reacts with readily accessible primary amines to form a covalent secondary amine bond Can be stored for at least 12 months at -20°C Consistent conjugation yields reliable conjugates Enzyme activity is 120-200 units/mg