Epredia™ Lab Vision™ UltraVision™ Detection System: anti-Polyvalent

Description

• This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, enzyme-labeled streptavidin, and substrate/chromogen
• Labeled streptavidin-biotin immunoenzymatic antigen detection system Species: Goat Specificity: anti-Mouse IgG (H+L), anti-Rabbit IgG (H+L) Enzyme: Alkaline Phosphatase Chromogen/Substrate: Fast-Red/Naphthol Phosphate Buffer Includes: TP-015-AF: 15mL Ultra V Block (TA-015-UB), 15mL Biotinylated Goat Anti-Polyvalent (TP-015-BN), 15mL Streptavidin Alkaline Phosphatase (TS-015-AP), 15mL Naphthol Phosphate Substrate (TA-015-AFS), three Fast-Red Tablets (TA-003-AFC) Note: UltraVision detection system detects a specific antibody bound to an antigen in tissue sections
• The specific antibody is located by a biotin-conjugated secondary antibody
• This step is followed by the addition of a streptavidin-enzyme conjugate that binds to the biotin present on the secondary antibody
• The specific antibody, secondary antibody, and streptavidin-enzyme complex is then visualized with an appropriate substrate/chromogen.