TP060AL

Epredia™ Lab Vision™ UltraVision™ Detection System: anti-Polyvalent, AP (Ready-To-Use)

Manufacturer: Fischer Scientific

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Chromagen

None provided

Enzyme

Alkaline Phosphatase

Species

Goat

Substrate

None provided

Quantity

60 mL

Count

300-600 slides

Product Line

Lab Vision™, UltraVision™

Specificity

anti-Mouse IgG (H+L), anti-Rabbit IgG (H+L)

Includes

TP-060-AL: 60mL Ultra V Block (TA-060-UB), 60mL Biotinylated Goat Anti-Polyvalent (TP-060-BN), 60mL Streptavidin Alkaline Phosphatase (TS-060-AP)

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Description

  • This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, enzyme-labeled streptavidin, and substrate/chromogen
  • Labeled streptavidin-biotin immunoenzymatic antigen detection system Species: Goat Specificity: anti-Mouse IgG (H+L), anti-Rabbit IgG (H+L) Enzyme: Alkaline Phosphatase Chromogen/Substrate: None provided Includes: TP-060-AL: 60mL Ultra V Block (TA-060-UB), 60mL Biotinylated Goat Anti-Polyvalent (TP-060-BN), 60mL Streptavidin Alkaline Phosphatase (TS-060-AP) TP-125-AL: 125mL Ultra V Block (TA-125-UB), 125mL Biotinylated Goat Anti-Polyvalent (TP-125-BN), 125mL Streptavidin Alkaline Phosphatase (TS-125-AP) Note: UltraVision detection system detects a specific antibody bound to an antigen in tissue sections
  • The specific antibody is located by a biotin-conjugated secondary antibody
  • This step is followed by the addition of a streptavidin-enzyme conjugate that binds to the biotin present on the secondary antibody
  • The specific antibody, secondary antibody, and streptavidin-enzyme complex is then visualized with an appropriate substrate/chromogen.