Thermo Scientific™ N-PER™ Neuronal Protein Extraction Reagent

Description

• Thermo Scientific™ N-PER Neuronal Protein Extraction Reagent is a proprietary cell lysis reagent optimized for efficient extraction of proteins from all cellular compartments of neuronal tissue and primary cultured neurons
• Neuron cell lysates prepared with the N-PER Reagent may be used for enzymatic activity assays (e.g., Phosphatase, Kinase, ATPase Assays), immunoassays (e.g., Western blots, ELISAs, RIAs) and protein purification
• The N-PER Lysis Reagent enables protein extraction to be completed in less than 30 minutes and requires mechanical disruption of tissue (e.g., Dounce Homogenization, Polytron)
• Typical neuronal protein yields are 70-90μg of protein per mg of brain tissue or 300μg of total protein from 10 6 primary neuronal cells
• Highlights: Optimized – efficient extraction of total neuronal protein, including membrane proteins, from tissue or primary cultured cells Gentle – preserves protein function without compromising yield Versatile – can be supplemented with protease inhibitors, reducing or chelating agents or required cofactors Compatible – extracts are suitable for use with total protein, enzymatic and immunological assays and protein purification methods Recommended for: Isolate functional proteins from neuronal tissues or primary neuronal cells; Study enzymatic activity assays specific to neuronal tissue; Generate lysates from neuronal tissues for biomarker analysis