Thermo Scientific™ Syn-PER™ Synaptic Protein Extraction Reagent

Description

• Thermo Scientific™ Syn-PER Synaptic Protein Extraction Reagent is a proprietary cell lysis reagent for efficient isolation of synaptosomes containing functional synaptic proteins from neuronal tissue and primary cultured neurons
• Syn-PER Reagent is used to prepare synaptosomes containing biologically active pre-and post-synaptic proteins
• When used with fresh neuronal tissue or primary cultured neurons, synaptosomes prepared with the Syn-PER Reagent can be used to study synaptic transmission
• The synaptosomal proteins contained in these extracts can also be used for downstream applications such as Western blotting, immunoprecipitation, enzymatic activity assays and protein-protein interaction studies
• Syn-PER Synaptic Protein Extraction Reagent minimizes the degradation of phosphoproteins and is ideal for studies requiring the preservation of phosphoprotein integrity
• Highlights: Efficient extraction – obtain up to 10μg of synaptic protein per milligram of neuronal tissue or 4μg synaptic protein per 35mm dish of primary cultured neurons (10 6 cells) Gentle formulation – isolate viable synaptosomes, extract native synaptic proteins and preserve phosphoprotein integrity Fast procedure – obtain synaptosomal suspension in less than one hour Simple protocol – requires no ultracentrifugation steps Recommended for: Isolate functional synaptosomes to study neurotransmitter release; Extract pre- and post-synaptic proteins to identify changes in protein composition and function in synapses; Preserve and study labile or transient protein phosphorylation events associated with synapses