Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Description

• Thermo Scientific™ NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours
• The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions
• A benchtop microcentrifuge, tubes and pipettors are the only tools required
• The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA
• Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays
• Highlights: Fast – obtain nuclear and cytoplasmic fractions in less than two hours Proven – the NE-PER Kit is referenced in more than 950 distinct publications Scalable – two kit sizes for producing extracts from cells and tissues Convenient – simple instructions do not require ultracentrifugation over gradients Compatible – use for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays Includes: Two kit sizes, each containing three buffer and detergent solutions Compatible with: Thermo Scientific LightShift Chemiluminescent EMSA Kit (Mfr
• No
• 20148) Storage Requirements: Upon receipt store kit components at 4°C