Thermo Scientific™ Mitochondria Isolation Kit for Tissue

Description

• Isolate intact mitochondria from soft and hard tissue samples in about an hour with Thermo Scientific™ Mitochondria Isolation Kit for Tissue
• The kit provides for two methods for mitochondria isolation
• The first method uses a unique reagent-based procedure that enables simultaneous multi-sample processing
• The second method relies on traditional Dounce homogenization for tissue disruption and subsequent isolation of the organelle
• Both procedures use differential centrifugation to separate the intact mitochondria using a bench-top microcentrifuge and are completed in less than 60 minutes
• In addition, both procedures have been optimized for maximum yield of mitochondria with minimal damage to integrity
• The isolated mitochondria may be used for a number of downstream applications, including 1D and 2D Western blotting and protein profiling using mass spectroscopy
• Highlights: Quick and convenient – isolate intact mitochondria in less than 60 minutes Versatile – offers two methods of isolation from both soft- and hard-tissue samples Multi-sample format – reagent-based approach enables simultaneous processing of multiple samples Optional alternate method – reagents and protocol included for the traditional Dounce homogenization procedure with fewer required strokes Benchtop-compatible – both procedures are performed using a microcentrifuge tube Includes: Mitochondria Isolation Reagents A, B and C; bovine serum albumin and a phosphate-buffered saline pack