Thermo Scientific™ DMEM for SILAC

Description

• Thermo Scientific™ DMEM for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS)
• Features of DMEM for SILAC: Flexible —liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling High quality —medium is sterile, endotoxin-free, and cell culture-compatible SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples
• This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins
• This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis
• SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells
• Related products NeuCode™ Lysine -080, 50 mg (A33613) Fetal Bovine Serum, dialyzed, US origin (26400036) RPMI 1640 Media for SILAC (88365)