Thermo Scientific™ RPMI 1640 Medium for SILAC

Description

• Thermo Scientific RPMI Media for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS)
• Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples
• This approach entails the in vivo metabolic incorporation of “heavy” 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins
• This SILAC media is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis
• SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells
• Features of RPMI Media for SILAC: Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling High quality—media are sterile, endotoxin-free and cell culture-compatible