Description

• Application: Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.
• Biochem/physiol Actions: β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
• Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.
• Physical properties: Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
• Unit Definition: 1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C
• Other Notes: Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590)[1]; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique[2]; Enzyme immunoassay of thyrptropin releasing hormone (TRH)[3]; Preparation of enzyme-antibody conjugates[4]