Concanavalin A from Canavalia ensiformis (Jack bean)

Description

• Application: Detects glycoproteins containing α-D-mannose, α-D-glucose when used with appropriate peroxidase substrate
• Biochem/physiol Actions: Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
• Packaging: Package size based on protein content
• Unit Definition: One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
• Physical form: Lyophilized powder containing tris-citrate buffer salts and trace calcium and manganese
• Preparation Note: Prepared from peroxidase type VI (P8375), using the method of Wilson and Nakane,[1] which promotes conjugation but prevents the interaction between Con A and peroxidase sugar residues.
• Analysis Note: Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.