4-Nitrophenyl phosphate disodium salt hexahydrate
chromogenic, tablet
Manufacturer: Sigma Aldrich
CAS Number: 333338-18-4
Synonym(S): p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 TABS | N2640-50-TABS | In Stock | ₹ 36,859.13 |
| 100 TABS | N2640-100-TABS | In Stock | ₹ 57,653.95 |
N2640 - 50 TABS
In Stock
Quantity
1
Base Price: ₹ 36,859.13
GST (18%): ₹ 6,634.643
Total Price: ₹ 43,493.773
product name
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
form
tablet
Quality Level
200
solubility
water: 1 tablet/3 mL, clear, colorless
storage temp.
−20°C
SMILES string
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key
KAKKHKRHCKCAGH-UHFFFAOYSA-L
Other Options
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Description
- General description: Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
- Application: 4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase.[1] It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.[2]
- Biochem/physiol Actions: 4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol.[3] pNPP is a common chromogenic substrate for alkaline phosphatase.[2]
- Quantity: Contains 15 mg substrate per tablet
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves