N3755

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

Manufacturer: Sigma Aldrich

CAS Number: 9013-53-0

Synonym(S): Micrococcal nuclease, Endonuclease micrococcal, MNase, Micrococcal endonuclease

Select a Size

Pack Size SKU Availability Price
50 UNITS N3755-50-UNITS In Stock ₹ 17,693.40
200 UNITS N3755-200-UNITS In Stock ₹ 49,528.20
500 UNITS N3755-500-UNITS In Stock ₹ 98,767.80

N3755 - 50 UNITS

₹ 17,693.40

In Stock

Quantity

1

Base Price: ₹ 17,693.40

GST (18%): ₹ 3,184.812

Total Price: ₹ 20,878.212

biological source

Staphylococcus aureus

Quality Level

300

form

lyophilized powder

specific activity

100-300 units/mg protein

composition

Protein, ≥40% E1%/280 (Balance primarily sodium citrate)

technique(s)

DNA purification: suitable

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

Other Options

Image Product Name Manufacturer Price Range
Sigma Aldrich Fine Chemicals Biosciences Nuclease micrococcal from Staphylococcus aureus 100-300 units/mg protein | 9013-53-0 | MFCD00131731 | 500UN
-- ₹ 1,00,153.11
9013-53-0 | Micrococcal endonuclease
-- --

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Description

  • Application: Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. [1] It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases. [2]
  • Biochem/physiol Actions: Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C. [3]
  • Unit Definition: One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.
  • Other Notes: Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves