Description

• Application: Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Product P7125 is provided in powder form. Product P7125 has been used to denature DNA from kidney cells[1] and to digest pathology samples from anal canal carcinomas (ACC) biopsies prior to EGFR staining[2].
• Biochem/physiol Actions: It does not cleave at valine, alanine, or glycine linkages.[5] Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion.[5][6] Pepsin is inhibited by several phenylalanine-containing peptides.[7]
• Unit Definition: One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)
• Analysis Note: Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
• Other Notes: View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.