6911011
Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
Manufacturer: Fischer Scientific
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Product Type
Substrate
Form
Liquid
Product Line
SuperSignal™
Substrate
Horseradish Peroxidase
Signal Duration
6 to 24 hr.
Detection Method
Chemiluminescence
For Use With (Application)
Western Blot
Conjugate
HRP
Recommended Antibody Concentrations
2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Shipping Condition
Room Temperature
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Description
- Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis
- SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include: Sensitivity: pictogram to femtogram sensitivity Stability: 8-hr working solution stability; 1-year kit stability at room temperature Compatibility: nitrocellulose and PVDF membranes Signal duration: 6 to 24 hours Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL) Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL) SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates
- The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images
- SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.