Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Description

• Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting
• SuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme
• The substrate kit enables detection of low femtogram (that's mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies
• When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates
• Highlights: ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes Quantitative – produces quantitative signal that is measurable over two orders of magnitude Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks) Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system Excellent signal duration – 8 hours of usable light-output when conditions are optimized Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature