Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin
• Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes
• Use the isotype control at the same concentration as the experimental antibody
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer for more information
• Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company
• Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications
• It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC)
• Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains
• It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.