7218607
Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 506, eBioscience™, Invitrogen™
Rat Isotype Control
Manufacturer: Fischer Scientific
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Antigen
Rat IgG2a kappa
Concentration
0.2 mg/mL
Conjugate
eFluor 506
Host Species
Rat
Target Species
Not Applicable
Gene Alias
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Primary or Secondary
Isotype Control (Primary)
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
eBR2a
Applications
Control, Flow Cytometry
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Isotype
IgG2a κ
Purification Method
Affinity chromatography
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Description
- Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin
- Applications Reported: This eBR2a antibody has been reported for use in flow cytometric analysis
- Applications Tested: This eBR2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes
- Use the isotype control at the same concentration as the experimental antibody
- eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm
- We recommend using a 510/20 band pass filter, or equivalent
- Please make sure that your instrument is capable of detecting this fluorochrome
- Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser
- Filtration: 0.2 μm post-manufacturing filtered
- Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications
- It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC)
- Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains
- It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.