7091165

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™, Invitrogen™

Rat Isotype Control

Manufacturer: Fischer Scientific

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Antigen

Rat IgG2a kappa

Concentration

0.2 mg/mL

Conjugate

eFluor 450

Host Species

Rat

Target Species

Not Applicable

Gene Alias

IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2

Primary or Secondary

Isotype Control (Primary)

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Clone

eBR2a

Applications

Control, Flow Cytometry

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Isotype

IgG2a κ

Purification Method

Affinity chromatography

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Description

  • Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin
  • Applications Reported: Rat IgG2a Isotype Control eFluor® 450 has been reported for use in flow cytometric analysis
  • Applications Tested: This Rat IgG2a Isotype Control has been tested by flow cytometric analysis of mouse splenocytes
  • Use isotype control at the same concentration as experimental antibody
  • Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications
  • It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC)
  • Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains
  • It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.