Fluorescein/Oregon Green Polyclonal Antibody, Alexa Fluor™ 488, Invitrogen™

Description

• We offer a variety of anti-fluorescent dye antibodies that recognize specific fluorophores and, in most cases, quench their fluorescence
• Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria, or other biomolecules have been internalized by endocytic or pinocytic processes
• In addition to their utility in quenching the fluorescence of extracellular fluorescein, the high affinity of Invitrogen's anti-fluorescein antibodies for both fluorescein and the structurally related Oregon GreenTM dye makes them ideal for immunochemical applications
• Harmer and Samuel report the fluorescein-anti-fluorescein system provides a sensitive alternative to the biotin-streptavidin methods conventionally used for enzyme-linked immunosorbent assays (ELISAs)
• Fluorescein has also been found to be an excellent hapten for FISH, yielding sensitive results with extremely low background levels
• The Alexa Fluor 488 conjugate can be used to amplify the fluorescence signal while still allowing fluorescein-compatible optics
• The approximate absorption (Abs) and fluorescence emission (Em) maxima in nm: 495/519
• Our anti-dye antibodies can be used in many different applications
• It is a good practice to centrifuge the labeled antibody solutions briefly in a microcentrifuge before use; only the supernatant should then be added to the experiment
• Fluorescein/Oregon Green Polyclonal antibody specifically detects Fluorescein/Oregon Green in Chemical samples
• It is validated for ELISA.