Invitrogen™ LIVE/DEAD™ Fixable Green Dead Cell Stain Kit, for 488 nm excitation

Description

• Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation Kit has been optimized and validated for use with a blue laser flow cytometer Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs Optimal brightness The LIVE/DEAD™ Fixable Green stain was selected based on its fluorescent properties to provide a bright signal when excited with a blue 488 nm laser The green-fluorescent reactive dye has an excitation maximum of ∼495 nm making it ideal for use with the blue laser and an emission of ∼520 nm allowing collection in the FITC channel Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments