Thermo Scientific™ CellSensor™ CYP1A1-blaLS-180 Cell Line

Description

• CYP1A1 is an extrahepatic cytochrome P450 with broad substrate specificity involved in the metabolism of numerous potentially toxic and carcinogenic compounds
• This cell-based assay measures CYP1A1 induction via the aryl hydrocarbon receptor (AhR) pathway
• CYP1A1 induction is regulated by AhR, a transcription factor existing in a multiprotein complex in the cytoplasm
• Ligands bind AhR in the cytoplasm of the cells, whereupon the AhR-ligand complex translocates to the nucleus and forms a heterodimer with AhR nuclear translocator (Arnt)
• This complex then binds the dioxin response element (DRE) in the 5' upstream region of the CYP1A1 promoter, enhancing CYP1A1 expression
• The GeneBLAzer™ CYP1A1-bla LS-180 cell line contains a beta-lactamase reporter gene whose expression is driven by the CYP1A1 promoter, which serves as a readout for CYP1A1 expression
• This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time
• The Z' and EC50 concentrations of 2, 3, 7, 8- tetrachlorodibenzo-p-dioxin (TCDD) were determined
• Other CYP1A1 inducers include synthetic and natural chemicals including halogenated and polycyclic aromatic hydrocarbons, and dietary compounds such as flavonoids
• Additional testing information using known activators of the pathway are also provided
• Academic and non-profit customers, please inquire for special pricing.