K1679B
Thermo Scientific™ LanthaScreen™ ATF2 (19-106) A549 Cell Line
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1679B-Each-of-1 | In Stock | ₹ 9,05,224.80 |
K1679B - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,05,224.80
GST (18%): ₹ 1,62,940.464
Total Price: ₹ 10,68,165.264
Cell State
Dividing Cells
Quantity
1 Vial
Druggable Target
Signaling Pathway
Content And Storage
LanthaScreen™ Cellular assays are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
System Type
LanthaScreen™
Related Products
Description
- The LanthaScreen™ATF2 (19-106) A549 cell line contains a fusion protein consisting of GFP and a fragment encoding for aa 19-106 of ATF2 under the control of a CMV promotor
- A549 is a lung carcinoma cell line which shows inducible activation of JNK by number of different ligands, such as TNF and EGF, which leads to the transient phosphorylation of GFP-ATF2 (19-106)
- This LanthaScreen™cell line therefore allows the analysis of JNK activity using GFP-ATF2 phosphorylation as readout
- The GFP-ATF2 (19-106) construct was transfected into A549 cells using Lipofectamine™2000, followed by selection with Blasticidin
- This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker
- This cell line is validated for EC50 and Z' under optimized conditions using TNF as ligand for JNK mediated GFP-ATF2 (19-106) phosphorylation
- This cell line has been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time.