K1817
Thermo Scientific™ CellSensor™ ESRE-blaHeLa Cell Line
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1817-Each-of-1 | In Stock | ₹ 12,81,688.80 |
K1817 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 12,81,688.80
GST (18%): ₹ 2,30,703.984
Total Price: ₹ 15,12,392.784
Alternate Agonists
Thapsigargin
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
CellSensor™ ESRE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application)
FRET
Human Protein Class
Cell Signalling
Product Line
CellSensor™
Readout
End Point
Target Entry
ATF6, ER stress response pathway
System Type
CellSensor™
Antagonists
Salubrinal
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
ESRE
Primary Agonist
Tunicamycin
Quantity
1 Vial
Reporter Gene
BLA (Beta-Lactamase), BLA (Beta-lactamase)
Cell Line
HeLa
Druggable Target
Signaling Pathway
Related Products
Description
- Endoplasmic Reticulum (ER) stress is associated with a variety of pathophysiological conditions, such as neurodegenerative diseases, diabetes, tumor growth under hypoxic conditions, and ischemic heart disease
- Proteins in the ER misfold or unfold, and accumulate under stress conditions, which promote the expression of ER stress responsive genes
- One of the mechanisms for mediating ER stress response is the activation of transcription factor ATF6
- The quiescent form of ATF6 (p90ATF6), a type II-transmembrane protein, is embedded in the ER membrane and proteolyzed in an ER stress-dependent manner
- The liberated N-terminal fragment (p50ATF6) translocates to the nucleus, binding to ER stress response element (ERSE) present in the proximal promoter regions of many ER stress-responsive proteins including ER chaperones
- To better understand the pathological processes and provide novel avenues to potential therapies, ESRE–bla HeLa cells are engineered to express beta-lactamase under the control of ER stress response element
- This is a clonal population isolated by FACS and its dose response curves with tunicamycin and thapsigargin are performed
- This cell line also response to other known ER stress inducers.