K1838
Thermo Scientific™ CellSensor™ Gli-bla22Rv.1 Cell Line
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K1838-Each-of-1 | In Stock | ₹ 9,05,224.80 |
K1838 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 9,05,224.80
GST (18%): ₹ 1,62,940.464
Total Price: ₹ 10,68,165.264
Antagonists
KAAD-Cyclopamine
Cell State
Dividing Cells
Detection Method
Fluorescence
Gene Symbol
GLI
Primary Agonist
Constitutive
Quantity
1 vial
Reporter Gene
BLA (Beta-Lactamase), BLA (Beta-lactamase)
Type
Cell Line
System Type
CellSensor™
Assay Entry
Cell-based beta lactamase reporter gene
Content And Storage
CellSensor™ Gli-bla 22Rv.1 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
For Use With (Application)
FRET
Human Protein Class
Cell Signalling
Product Line
CellSensor™
Readout
End Point
Target Entry
SMO, Hedgehog pathway
Cell Line
22Rv.1
Druggable Target
Signaling Pathway
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Description
- The morphogenic signal Shh provides in the developing CNS induces proliferation of neuronal precursor cells in the developing cerebellum and other tissues
- Proliferative signaling by Shh is involved in the development of cancer, including specific brain and skin cancers such as basal cell carcinomas
- Signaling takes place through a Patched (PTC-1)/Smoothened (SMO) Receptor complex
- The activation of Patched by Shh releases the inhibition of Patched on Smoothened leading to Sonic Hedgehog pathway activation of the Gli transcription factor to induce downstream gene expression
- The CellSensor™ Gli-bla 22Rv1 cell line contains a beta-lactamase reporter gene under control of the Gli response element stably integrated into 22Rv1 cells
- This cell line is a clonal population isolated by flow cytometry
- The clone was selected based on inhibition of the pathway with KAAD-Cyclopamine
- This cell line has also been tested for assay performance under variable conditions, including DMSO concentration, cell number, compound incubation time, and substrate loading time and validated for Z' under maximal inhibition of β-lactamase activity with Clavulanic Acid.