Description

• General description: Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface.[1] There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34kDa and they possess a conserved sequence of 120 amino acids.[2]
• Application: A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
• Biochem/physiol Actions: Fibroblast growth factors (FGF) actively participate in cellular responses including embryogenesis[2] and organogenesis.[1] It specifically controls cell proliferation, differentiation and migration. FGF is known to regulate nervous system functions, tissue repair mechanism and wound healing. It also controls angiogenesis of tumor cells.[2] Abnormal FGF mediated signaling induces tumor angiogenesis, by stimulating cancer cell proliferation and survival.[1] FGFs carries out its function by mediating through four receptor tyrosine kinases : FGF-receptors FGFR1, FGFR2, FGFR3 and FGFR4.[2]
• Quality: Although the major mitogenic component of this product is FGF2, the activity per unit weight of protein is less than that of purified FGF2.
• Physical form: Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.1 mg/ml BSA.
• Preparation Note: Purified glands by a modification of the method of Gospodarowicz.
• Analysis Note: The biological activity is measured using a 3-day MTT assay.