NGF-β from rat

Description

• General description: NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells.[1][2] It is a neurotrophin and belongs to the NGF family.[3]
• Application: NGF-β from rat is used for the following applications:In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)[4][5]Western blotting analysis[6]For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)[7]In cell culture and treatment (for neuronal differentiation)[8]In the preparation of stock solution[9]
• Biochem/physiol Actions: NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers.[3] It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same.[1] It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.[2]
• Physical form: Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine
• Analysis Note: The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.