H3662

HEPES sodium salt solution

Manufacturer: Sigma Aldrich

CAS Number: 75277-39-3

Select a Size

Pack Size SKU Availability Price
100 ML H3662-100-ML In Stock ₹ 6,782.10
500 ML H3662-500-ML In Stock ₹ 17,993.10
1 L H3662-1-L In Stock ₹ 30,125.40

H3662 - 100 ML

₹ 6,782.10

In Stock

Quantity

1

Base Price: ₹ 6,782.10

GST (18%): ₹ 1,220.778

Total Price: ₹ 8,002.878

Quality Level

200

sterility

sterile-filtered (0.2 μm filtered)

product line

BioReagent

form

solution

concentration

0.90-1.10 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested DNase, RNase. Protease, Nickase, free endotoxin, tested<5 ppm Heavy metals (as Pb)

cation traces

Fe: <5 ppm

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

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Description

  • General description: HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966.[1] It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.
  • Application: HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts.[2] It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.[3]

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves