Description

• General description: HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.[1]
• Application: HEPES has been used:To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines[4][5]As a component of platelet suspension buffer[6]To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue[7]As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay[8]For the adjustment and maintenance of pH of biological solutions[8]As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development[9]For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells[10]As a component of keratinocyte and fibroblast culture medium[11]
• Features and Benefits: Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applicationsHighly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °CNegligible metal ion bindingLess toxic to cells than other buffers such as Tris and phosphateStable in a wide pH rangeTested for Endotoxins and Total Aerobic Microbial CountFree from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease
• Other Notes: For additional information on our range of Biochemicals, please complete this form.