Description

• General description: HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]
• Application: HEPES has been used:As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo[3]As a component of Hanks′ solution used in the degradation testing of Iron[4]As a component of HEPES medium in the transfection of cell culture with plasmids[5]Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells[6]As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies[7]
• Features and Benefits: High purity product for biochemical and biological researchSuitable for Cell cultureHighly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
• Other Notes: Go to Redi-Dri™ Product Listing RDD002Easily compare specifications for HEPES products with the HEPES specification table.
• Legal Information: Redi-Dri is a trademark of Sigma-Aldrich Co. LLC