Thermo Scientific™ GeneJET Genomic DNA Purification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERK0722-Each-of-1 | In Stock | ₹ 69,132.48 |
FERK0722 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 69,132.48
GST (18%): ₹ 12,443.846
Total Price: ₹ 81,576.326
Column Type
Spin Column
Isolation Technology
Silica Spin Column
Scale
Mini
Final Product Type
gDNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Yield
≤30 μg
Content And Storage
• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening• 55 mL Digestion Solution; room temperature• 2 x 60 mL Lysis Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Sample Type
Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples
Elution Volume
200 μL
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity
250 Preps
Test Time
20 min. (After sample homogenization)
Starting Material Amount
Bacteria: ≤109cellsCells: ≤106Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse)Tissue: ≤20 mgWhole Blood: ≤200 μLYeast: ≤108cells
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Description
- Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast
- The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
- The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size
- Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions
- Highlights • Universal —can be used for both cell and tissue samples from a wide range of sources • Efficient —high yields of high molecular weight genomic DNA • Fast —20 minute procedure following cell lysis • Convenient —spin columns are capped and assembled with collection tubes Applications Isolated genomic DNA is ideal for use in common molecular biology procedures, including: • PCR and qPCR • FastDigest and conventional restriction digestion • Southern blotting