Invitrogen™ PureLink™ Genomic DNA Mini Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K182002-Each-of-1 | In Stock | ₹ 79,228.56 |
K182002 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 79,228.56
GST (18%): ₹ 14,261.141
Total Price: ₹ 93,489.701
Content And Storage
• 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature• 45 mL PureLink Genomic Digestion Buffer; room temperature• 50 mL PureLinkGenomic Wash Buffer 1; room temperature• 37.5 mL PureLink Genomic Wash Buffer 2; room temperature• 50 mL PureLink Genomic Elution Buffer; room temperature• 5 mL RNase A (20 mg/mL); room temperature• 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage• 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature• 5 x 100 PureLink Collection Tubes; room temperature
Sample Type
Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
Elution Volume
25 to 200 μL
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity
250 Preps
Test Time
Up to 15 min. after cell lysis
Starting Material Amount
Bacteria: ≤109cellsCells: ≤106Tissue: ≤25 mgMouse Tail: 1 cmRat Tail: 0.5 cmWhole Blood: ≤1 mLYeast: ≤107cellsFFPE: ≤8 sections of 5-15 μM thicknessOragene™ Preserved Saliva: ≤4 mLBlood Spots: ≤5 punches
Isolation Technology
Silica Spin Column
Scale
Mini
Final Product Type
gDNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Yield
≤40 μg
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Description
- The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types
- Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format
- Features of the PureLink™ Genomic DNA Mini Kit include: • Kit flexibility —one kit works with a variety of sample types and sizes • Ultraclean gDNA —minimal contamination of DNA product means successful downstream application • Improved design —optimized spin-column design and buffer formulation for better yield and purity Multiple sample sources, one kit The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual
- Optimized components and protocols The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use
- Columns are newly configured to bind and release DNA more efficiently
- Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination
- A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt
- DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage
- Higher yields and purity With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A 260 /A 280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells
- The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A 260 /A 280 and A 260 /A 230 , indicating no detectable contamination from carryover of protein or guanidine
- Order Info Shipping Condition: Room Temperature