Thermo Scientific™ GeneJET Whole Blood Genomic DNA Purification Mini Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERK0782-Each-of-1 | In Stock | ₹ 72,726.00 |
FERK0782 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 72,726.00
GST (18%): ₹ 13,090.68
Total Price: ₹ 85,816.68
Column Type
Spin Column
Isolation Technology
Silica Spin Column
Scale
Mini
Final Product Type
gDNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Shipping Condition
Room Temperature
Yield
≤65 μg
Content And Storage
• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening• 120 mL Lysis Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Sample Type
Bone Marrow, Buffy Coat, Dried Blood Spots, Buccal Swab, Urine, Whole Blood
Elution Volume
50 to 200 μL
For Use With (Application)
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Quantity
250 Preps
Test Time
20 min. (After sample homogenization)
Starting Material Amount
Bone Marrow: ≤200 μLBuccal Swabs: 1Buffy Coat: 200 μLDried Blood Spots: variesUrine: ≤4.5 mLWhole Blood: ≤500 μL
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Description
- Description GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
- The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size
- Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions
- Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
- The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size
- Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions
- Highlights • Efficient —high yields of high molecular weight genomic DNA • Fast —20 minute procedure following cell lysis • Convenient —spin columns are capped and assembled with collection tubes • Pure —DNA pure from enzyme inhibitors, RNA and proteins
- Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests • Compatible —suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluids Applications • Isolated genomic DNA is ideal for use in common molecular biology procedures, including: • PCR and qPCR • FastDigest and conventional restriction digestion • Southern blotting Includes Proteinase K Solution Lysis Solution Wash Buffer WB I (concentrated) Wash Buffer II (concentrated) Elution Buffer GeneJET Purification Columns (pre-assembled with collection tubes) Collection tubes Detailed Protocol