Invitrogen™ ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A11146-Each-of-1 | In Stock | ₹ 2,72,080.80 |
A11146 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,72,080.80
GST (18%): ₹ 48,974.544
Total Price: ₹ 3,21,055.344
Constitutive or Inducible System
Constitutive
Promoter
None (Promoterless), EF-1α, CMV
Selection Agent (Eukaryotic)
Blasticidin
Antibiotic Resistance Bacterial
Ampicillin (AmpR), Blasticidin (BsdR)
Gateway Enzyme
LR Clonase™ II Plus, LR Clonase™ II
Protein Tag Position (to your gene)
C-terminal
Selection Marker Promoter
PGK Promoter
Special Elements
WPRE, cPPT
Cloning Method
Gateway
Vector
pLenti
For Use With (Application)
Clone your own Promoter, Viral Expression
Delivery Type
Lentiviral
Product Type
Vector Kit
Content And Storage
pLenti6.4/R4R2/V5-DEST™ Kit• pLenti6.4/R4R2/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti6.4/CMV/V5-MSGW/lacZ plasmid (20 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/CMVp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/EF1αp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μgStore at -20°CpENTR 5' TOPO™ TA Cloning Kit(Cat. No. K95120)• pENTR 5' TOPO™ Reagents, store at -20°C.• One Shot™ TOP10 Chemically Competent E.coli (Cat. No. C404003), store at -80°C.One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C
Cleavage
No Cleavage Site
PCR Enzyme
Proof-Reading Polymerase
Replication Origin
pUC Origin
Selection Marker Eukaryotic
BsdR
Protein Tag
V5 Epitope Tag
Quantity
20 Reactions
Product Line
Gateway™, HiPerform™, ViraPower™
Related Products
Description
- The ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit contains the MultiSite Gateway™-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.4/R4R2/V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells
- The pLenti6.4/R4R2/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements
- Advantages Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells Easy, simultaneous, recombination-based cloning of multiple DNA fragments in a defined order and orientation using MultiSite Gateway™ technology Expression of the target gene under the control of a promoter of choice Stable, long-term expression Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems Key Features WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer
- WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements
- Promoterless vector to express the target gene under the control of a promoter of choice Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression Kit includes pLenti6.4/R4R2/V5-DEST™ Kit pENTR™ 5' TOPO™ TA Cloning Kit (Cat
- No
- K59120) One Shot™ Stbl3™ Chemically Competent E
- coli (Cat
- No
- C737303) pENTR™ Gus positive control plasmid