K36720

Invitrogen™ ViraPower™ II Lentiviral Gateway™ Expression System

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 K36720-Each-of-1 In Stock ₹ 2,44,273.80

K36720 - Each of 1

₹ 2,44,273.80

In Stock

Quantity

1

Base Price: ₹ 2,44,273.80

GST (18%): ₹ 43,969.284

Total Price: ₹ 2,88,243.084

Constitutive or Inducible System

Constitutive

Promoter

CMV

Selection Agent (Eukaryotic)

Blasticidin

Protein Tag

V5 Epitope Tag

Quantity

1 kit

Product Line

Gateway™, ViraPower™

Delivery Type

Lentiviral

Product Type

Lentiviral Expression System

Content And Storage

pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)• One Shot™ Stbl3™ Chemically CompetentE. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)• 50 mg of Blasticidin powder (Cat # R21001)• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.Storage• Vectors: -20°C• ViraPower™ Packaging Mix: -20°C• Blasticidin: -20°C• Lipofectamine™ 2000: 4°C (do not freeze)• One Shot™ Stbl3™ Chemically CompetentE. coli:-80°C• 293 FT Cells: Liquid Nitrogen

Cloning Method

Gateway

Vector

pLenti

For Use With (Application)

Viral Expression

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Description

  • Description Generates replication-incompetent lentivirus for transducing dividing and nondividing mammalian cells The pLenti6.2/V5-DESTvector in kit has CMV promoter for driving constitutive expression of target gene in mammalian cells Uses the PGK promoter instead of SV40 promoter in lentiviral vector for driving long-term, persistent expression of blasticidin stable selection marker Easy recombination-based cloning and stable expression, long term expression Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery