Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A11144-Each-of-1 | In Stock | ₹ 3,30,261.60 |
A11144 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,30,261.60
GST (18%): ₹ 59,447.088
Total Price: ₹ 3,89,708.688
Constitutive or Inducible System
Inducible
Inducing Agent
Doxycycline, Tetracycline
Product Type
Vector Kit
Content And Storage
ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit• pLenti6.3/TO/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C and protect from light.Store at -20°C unless otherwise notedOne Shot™ Stbl3™ Chemically CompetentE. coli(Cat. No. C737303)• Stbl3™ Cells in 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C.
Cleavage
No Cleavage Site
PCR Enzyme
Proof-Reading Polymerase
Replication Origin
pUC Origin
Selection Marker Eukaryotic
BsdR
Protein Tag
V5 Epitope Tag
Quantity
20 Reactions
Product Line
Gateway™, HiPerform™, T-REx™, ViraPower™
Delivery Type
Lentiviral
Promoter
CMV/TO
Selection Agent (Eukaryotic)
Blasticidin
Antibiotic Resistance Bacterial
Ampicillin (AmpR), Blasticidin (BsdR)
Gateway Enzyme
LR Clonase™ II Plus, LR Clonase™ II
Protein Tag Position (to your gene)
C-terminal
Selection Marker Promoter
PGK Promoter
Special Elements
WPRE, cPPT
Cloning Method
Gateway
Vector
pLenti
For Use With (Application)
Viral Expression
Related Products
Description
- The ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ tetracycline-regulated lentiviral expression vector, pLenti6.3/TO/V5-DEST™ for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells
- The pLenti6.3/TO/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements
- Advantages Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells Easy recombination-based cloning using Gateway™ technology Stable, long-term, tetracycline-regulated expression Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems Key Features WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer
- WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements
- Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene Blasticidin selection marker for stable selection under control of SV40 promoter Kit includes pLenti6.3/TO/V5-DEST™ vector pLenti3.3/TR vector pLenti6.3/TO/V5-GW/lacZ plasmid Tetracycline One Shot™ Stbl3™ Chemically Competent E
- coli (Cat
- No
- C737303) pENTRTM Gus positive control plasmid.