Invitrogen™ pBAD TOPO™ TA Expression Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K430040-Each-of-1 | In Stock | ₹ 1,69,066.56 |
K430040 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,69,066.56
GST (18%): ₹ 30,431.981
Total Price: ₹ 1,99,498.541
Constitutive or Inducible System
Inducible
Promoter
araBAD
Selection Agent (Eukaryotic)
None
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Cleavage
EK (Enterokinase) Recognition Site
Expression Mechanism
Cell-Based Expression
Cloning Method
TOPO-TA
Vector
pBAD
Inducing Agent
Arabinose
Product Type
TA Expression Kit
Content And Storage
Each pBAD TOPO™ TA Expression Kit contains two boxes and the LMG194E. colistab. The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (exceptTaqpolymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 μl aliquots of One Shot™ TOP10 Chemically CompetentE. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ cells at -80°C. Store the LMG194E. colistab at at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.
Bacterial or Yeast Strain
LMG194, TOP10
Expression System
E. coli
Protein Tag
V5 Epitope Tag
Quantity
40 Reactions
Product Line
TOPO™
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Description
- Linearized, topoisomerase I-activated vector for 5-minute cloning of Taq amplified PCR products araBAD promoter for tightly regulated expression in E
- coli V5 epitope tag for detection with Anti-V5 Antibody C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody Enterokinase cleavage site for removal of N-terminal leader peptide Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis