Invitrogen™ Tempus™ Spin RNA Isolation Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 43-802-04-Each-of-1 | In Stock | ₹ 57,325.20 |
43-802-04 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 57,325.20
GST (18%): ₹ 10,318.536
Total Price: ₹ 67,643.736
Content And Storage
• 50 RNA Purification Filters; room temperature• 2 x 100 Collection Tubes; room temperature• 2 x 80 mL 1X PBS; room temperature• 24 mL RNA Purification Resuspension Solution; room temperature• 2 x 22 mL RNA Purification Wash Solution 1; room temperature• 120 mL RNA Purification Wash Solution 2; room temperature• 4 x 1.9 mL Nucleic Acid Purification Elution Solution; room temperature
Purification Time
40 min.
Sample Type
Whole Blood
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
50 Preps
Starting Material Amount
Up to 3 mL of blood
Isolation Technology
Silica Spin Column
Elution Volume
100 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Shipping Condition
Room Temperature
Yield
≤25 μg
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Description
- This Applied Biosystems product is for the fast, easy, glass fiber filter-based isolation of RNA from blood using standard laboratory equipment in about one hour
- Contains sufficient reagents for RNA purification from 50 samples
- Gene expression profiling and analysis of whole blood is becoming an increasingly important life science tool
- Accurately measuring the molecular profile of blood-based RNA transcripts is a critical need in areas of research such as disease association studies, biomarker discovery and validation, and monitoring of drug treatment regimes
- Isolate high-quality RNA from blood, suitable for real-time PCR and microarray analysis Obtain highly stable RNA virtually free of RNase activity, contaminating gDNA, or RT-PCR inhibitors Easy workflow requires only standard laboratory centrifuges and ∼1 hour of time No separate RBC lysis or proteinase K treatment required Performance with Ease The ability of the Tempus system (Tempus Blood RNA Tubes and Tempus Spin RNA Isolation Kit) to stabilize RNA expression profiles has been tested over a range of storage conditions
- The combination provides stable RNA, high yields of purified RNA (observed yields of 6 to 25μg from a full blood draw from normal, healthy donors), and high-quality RNA (A260/280 ratio greater than 1.9 and RIN greater than 7.0) suitable for use in downstream applications such as quantitative PCR, microarray profiling, and other RNA analysis methods
- Accessory Product The highly unstable nature of RNA transcripts ex vivo creates an even greater challenge for accurate expression analysis
- Ongoing metabolic changes can drastically affect gene expression profiles in as little as one hour in standard blood collection tubes
- Use of the Applied Biosystems Tempus™ Blood RNA Tubes (Cat
- No
- 4342792) is therefore recommended
- These tubes are designed for direct collection of 3mL of blood into a plastic, evacuated blood collection tube containing RNA Stabilizing Reagent
- The RNA, and therefore the expression profile, is stable in the collection tube for up to five days at room temperature and a minimum of 7 days at 4°C Not high-throughput compatible (Manual)