Applied Biosystems™ MagMAX™-96 AI/ND Viral RNA Isolation Kit

Description

• The MagMAX™-96 AI/ND Viral RNA Isolation Kit is a first generation MagMAX™ Viral RNA Isolation Kit validated by the NVSL for use with AI/ND viruses
• Perfect for working with low viral concentrations, the kit contains sufficient reagents for 4 x 96 reactions
• Validated by the NVSL for use with AI/ND viruses Recover as few as 10 copies of viral RNA from 100 to 400 μL sample Typical viral RNA recovery of >50% Ideal when working with low viral concentrations Linear recovery down to 50 transcripts 96 samples can be processed in 30 minutes Easily integrated into robotic platforms Benefits of MagMAX™ Magnetic Bead-Based Purification: Magnetic beads offer many benefits compared to other technologies for isolating RNA
• Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields
• Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples
• Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20 to 50 μL of nuclease-free water, concentrating RNA from large, dilute samples
• Optimized for Isolation of Virus from Biofluids: The Applied Biosystems™ MagMAX™ Viral RNA Isolation Kit has been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media
• These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity
• Without the need for organic solvents or RNA precipitation, the MagMAX™ Viral RNA isolation kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields
• Get the Same Amount of RNA from 10-fold Less Sample The sensitivity of viral RNA detection was compared using RNA isolated with Applied Biosystems™ MagMAX™ protocol or the current National Veterinary Services Laboratories (NVSL) validated viral testing protocol
• Even at one-tenth the sample input, viral RNA isolated using the MagMAX™ kit could be detected at equal or greater levels than RNA isolated using the NVSL protocol and recommended competitor's kit
• Accessory Products: Carrier RNA (SKU#4382878), Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the MagMAX™ Viral RNA Isolation Kits that may also be purchased separately
• A 96-well magnetic ring stand is required to use this kit
• The Magnetic Stand-96 (SKU#AM10027) and the 96-well Magnetic Ring Stand (SKU#AM10050) are designed for paramagnetic bead precipitation from standard 96 well U-bottom microplates
• The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors
• For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended
• The MagMAX™ and MagMAX™-96 Viral RNA Isolation Kits (SKU#AM1939 and SKU#AM1836) are the newest generation MagMAX™ kits for this application, but are not yet validated by the NVSL.