Invitrogen™ MagMAX™-96 Blood RNA Isolation Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1837-Each-of-1 | In Stock | ₹ 59,549.76 |
AM1837 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 59,549.76
GST (18%): ₹ 10,718.957
Total Price: ₹ 70,268.717
Content And Storage
Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp.Store MagMax Turbo DNase Buffer and RNA Binding Beads at 4°C.Store Lysis/Binding Enhancer and TURBO DNase at -20°C.
Isolation Technology
Magnetic Bead
Final Product Type
Total RNA, Viral RNA
For Use With (Equipment)
MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions
96 Preps
Product Line
Ambion™, MagMAX™
Target
Viral DNA/RNA, Total RNA
Format
96-well Plate
Sample Type
Liquid Samples (e.g. Serum), Milk, Viral Samples, Whole Blood (Mammalian)
High-throughput Compatibility
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale
< 100 μL Blood Samples (Small-scale)
Starting Material Amount
Up to 50 μl whole blood
Yield
0.5 μg
Related Products
Description
- This Ambion™ kit is for the rapid, high-throughput purification of pathogen RNA from mammalian whole blood, milk, and other biofluids in 96-well plates
- RNA purified using the kit is ideal for RT-PCR applications
- The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions
- • Easily isolate viral or total RNA from whole blood • Save precious time—complete the protocol in less than 1 hour • Walk away—integrate with established robotic platforms • Validated with human, bovine, porcine, and ovine whole blood Benefits of MagMAX™ Magnetic Bead-Based Purification: Magnetic beads offer many benefits compared to other technologies for isolating RNA
- Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields
- Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples
- Since only a small volume of magnetic beads is needed for high- efficiency binding, the bound RNA can be eluted in just 30–50 μL of nuclease-free water, concentrating RNA from large, dilute samples
- Isolate RNA from as Little as 50 μl of Blood: For the highest-quality RNA, use whole blood samples that have not been frozen
- Approximately 300–500 ng of intact RNA can be consistently purified from 50 μl of human whole blood
- Bovine, porcine, and ovine whole blood samples can also be used
- Yields vary from species to species (see data)
- The recovered RNA is devoid of PCR inhibitors such as proteins and heme, commonly found in RNA isolated from blood
- Isolate Viral and Total RNA from Whole Blood Samples: The MagMAX™-96 Blood RNA Isolation Kit eliminates sample preprocessing, which can cause sample mix-up and cross-contamination
- The procedure is fast (less than 1 hour), simple, and well suited for automation
- The kit can also be used to process fewer than 96 samples at one time and is compatible with either manual processing using multi-channel pipettors or processing with robotic liquid handlers
- Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource
- The MagMAX™-96 Blood RNA Isolation Kit can also be used for total nucleic acid isolation by omitting the DNase treatment.