Applied Biosystems™ MagMAX™-96 Viral RNA Isolation Kit

Description

• Perfect for working with low viral concentrations, the kit contains sufficient reagents for 5 × 96 reactions
• Benefits of MagMAX™ Magnetic Bead-Based Purification: Magnetic beads offer many benefits compared to other technologies for isolating RNA
• Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields
• Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples
• Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50μL of nuclease-free water, concentrating RNA from large, dilute samples
• Optimized for Isolation of Virus from Biofluids: The Applied Biosystems™ MagMAX™ Viral RNA Isolation Kits have been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media
• These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity
• Without the need for organic solvents or RNA precipitation, the MagMAX™ Viral RNA Isolation Kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields
• With this kit, DNA and RNA input levels ranging from 20 copies to 25 x 10 6 copies have been quantitatively recovered from plasma, serum, and milk
• And even with less sample input, pathogen detection was as sensitive (equivalent C Ts ) or more sensitive (lower C Ts ) using nucleic acid isolated with the MagMAX™ kit when compared to nucleic acid isolated using another company's product
• Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource
• Accessory Products: Carrier RNA (SKU#4382878), Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the MagMAX™ Viral RNA Isolation Kits that may also be purchased separately
• A 96-well magnetic ring stand is required to use this kit
• The Magnetic Stand-96 (SKU#AM10027) and the 96-well Magnetic Ring Stand (SKU#AM10050) are designed for paramagnetic bead precipitation from standard 96- well, U-bottom microplates
• The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors
• For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended
• For an NVSL-validated version of this kit, please see the MagMAX™-96 AI/ND Viral RNA Isolation Kits (SKU#1929 and SKU#AM1835).